Description
Use kit to obtain up to 5-log enrichment of human epithelial tumor cells, directly from whole blood, bone marrow or PBMC
Tumor cell clumps can be enriched from bone marrow. Enriched cells are bead-free, viable and suitable for immunocytochemical staining or any other downstream application.
- High enrichment of viable human tumor cells
- Isolate directly from whole blood, bone marrow or PBMC
- Viable cells for any cellular applications
- Capacity: Processes approx. 4x109 cells total
- Cell Type: Epithelial Cells, Tumor Cells
- Isolation Method: Positive Isolation
- Output Viability: >95%
- Reactivity: Human
- Sample Type: Blood, PBMC
- Starting Material (Cell No.): 2x107 PBMCs per isolation
- Target Species: Human, Bull
- Starting Samples: Whole blood, bone marrow, buffy coat, PBMC
- Methodology: Add CELLection Dynabeads to the sample. The beads will bind the tumor cells after a short incubation. The bead-bound tumor cells are quickly separated on a magnet and subsequently released from the beads with the supplied Release Buffer. (CELLection Dynabeads contains a DNA linker that is cleaved by the DNase I in the Release Buffer.)
To deplete or use the isolated tumor cells for molecular applications, use the equivalent product Dynabeads Epithelial Enrich. This product contains the same antibody, but with no release of the cells from the beads after positive isolation. Cell Analysis, Cell Isolation, Cell Isolation and Expansion, Human Cell Isolation